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| 基于蝉翅的生物SERS基底用于快速灵敏检测三聚氰胺 |
| Rapid and sensitive detection of melamine using biological SERS substrate based on cicada wing |
| 投稿时间：2025-02-17 |
| DOI：10.3969/j.issn.1005-5630.202502170025 |
| 中文关键词: 生物材料 疏水性 表面增强拉曼光谱 三聚氰胺 |
| 英文关键词:biomaterials hydrophobicity surface-enhanced Raman spectroscopy melamine |
| 基金项目:国家自然科学基金（61875126） |
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| 中文摘要: |
| 许多生物材料表面具有独特的微纳米结构，是制备表面增强拉曼光谱（surface-enhanced Raman spectroscopy，SERS）基底的理想材料。将氧化石墨烯（graphene oxide，GO）和银纳米颗粒（AgNPs）依次修饰在疏水生物材料蝉翅（cicada wing，CW）表面，制备得到高性能的AgNPs-GO-CW SERS基底。以罗丹明6G（rhodamine 6G，R6G）作为探针分子，系统评估了该基底的SERS性能；并将其应用于实际检测场景，实现了对奶粉中三聚氰胺的快速无标记检测。结果表明，所制备的AgNPs-GO-CW基底增强因子高达1×1010，对R6G的检测限（limit of detection，LOD）低至10-13 mol/L，且具备优异的光谱均一性与批次重复性，二者对应的相对标准偏差分别为10.71%和4.57%。该基底无需额外功能修饰，即可直接提取奶粉中三聚氰胺的特征指纹光谱，对应的LOD低至2.5×10-7 mol/L。该制备方法可简单地扩展至蝴蝶翅膀、蜻蜓翅膀等其他生物材料。所制得的SERS基底具有成本低、绿色环保、柔性便携及灵敏度高等优势，可广泛应用于食品安全检测及环境监测等领域。 |
| 英文摘要: |
| Many biological materials possess unique micro-nano structures on their surfaces, making them attractive candidates for the fabrication of surface-enhanced Raman spectroscopy (SERS) substrates. In this study, we prepared a high-performance AgNPs-GO-CW substrate by sequentially modifying graphene oxide (GO) and silver nanoparticles (AgNPs) onto the hydrophobic biomaterial cicada wing (CW). Rhodamine 6G (R6G) was used as a probe molecule to evaluate the SERS performance of the substrate. For practical application, this substrate enabled the rapid label-free detection of melamine in milk powder. The results showed that the enhancement factor of the AgNPs-GO-CW substrate reached as high as 1010, the limit of detection (LOD) for R6G was as low as 10-13 mol/L, and the substrate exhibited excellent spectral uniformity and batch repeatability, with relative standard deviations of 10.71% and 4.57%, respectively. The fingerprint spectrum of melamine in milk powder could be directly acquired without additional functional modification, with the corresponding LOD as low as 2.5×10-7 mol/L. The preparation method could be readily extended to other biomaterials, such as butterfly wings and dragonfly wings. Owing to its advantages of low cost, environmental friendliness, flexibility, portability and high sensitivity, the as-prepared SERS substrate shows great potential for widespread applications in food safety and environmental monitoring. |
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