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| 飞秒激光三维光刻制备重组Ⅲ型人源化胶原蛋白水凝胶微结构 |
| Fabrication of recombinant humanized type Ⅲ collagen hydrogel microstructures via femtosecond laser 3D lithography |
| 投稿时间：2025-02-09 |
| DOI：10.3969/j.issn.1005-5630.202502090019 |
| 中文关键词: 飞秒激光加工 重组Ⅲ型人源化胶原蛋白 水凝胶 细胞培养支架 |
| 英文关键词:femtosecond laser processing recombinant human type Ⅲ collagen hydrogel cell culture scaffold |
| 基金项目:国家自然科学基金(62375174) |
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| 中文摘要: |
| 飞秒激光三维光刻是一种高精度、低损伤、非接触、无热化的微纳米制造技术，被广泛应用于生物材料和水凝胶等软物质微结构制备中。重组Ⅲ型人源化胶原蛋白（rhCol Ⅲ）以其高生物相容性和细胞黏附性能，广泛应用于细胞培养与组织工程。然而，传统方法制备的rhCol Ⅲ水凝胶加工精度低（百微米量级），很难实现与人体组织尺寸相近的结构制备，大大限制了生物材料和支架在仿生领域的应用，尤其是精密化结构对细胞生长、迁移、分化等各种活动的影响。为此，提出了一种利用800 nm近红外飞秒激光加工rhCol Ⅲ和亚甲基蓝（MB）光敏剂的混合水凝胶聚合成型的新方法。通过探索优化加工参数，成功制备了不同孔径和面积的支架阵列。实验表明，飞秒激光加工能够显著提高rhCol Ⅲ水凝胶支架的分辨率和结构精度，支架最小宽度可达3 μm。该方法在制备高精度仿生结构及其在细胞培养、血管化等方面具有重要的应用潜力。 |
| 英文摘要: |
| Femtosecond laser 3D lithography is a high-precision, low-damage, non-contact, and non-thermal micro/nano-fabrication technique, which is widely employed in creating microstructures of soft materials, such as biomaterials and hydrogels et al. Recombinant humanized type Ⅲcollagen (rhCol Ⅲ) has been extensively used in cell culture and tissue engineering owing to its excellent biocompatibility and cell adhesion properties. However, rhCol Ⅲ hydrogels produced via conventional methods exhibit low processing precision with hundreds of microns, making it difficult to fabricate microstructures with dimensions comparable to human tissues. This limitation significantly hampers the application of biomaterials and scaffolds in biomimetic research, particularly investigating the effects of precise structures on cell growth, migration, and differentiation. To overcome this challenge, we propose a novel method that polymerizes a composite hydrogel of rhCol Ⅲ and methylene blue (MB) photosensitizer using 800 nm near-infrared femtosecond laser processing. By optimizing the processing parameters, scaffold arrays with various pore sizes and areas were successfully fabricated. Experimental studies reveal that femtosecond laser fabrication substantially enhances both resolution and structural fidelity of rhCol Ⅲ hydrogel scaffolds, achieving a minimum structural width of 3μm. This methodology demonstrates promising potential for engineering high-precision biomimetic constructs, particularly in applications requiring controlled cellular culture environments and advanced vascularization processes. |
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