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| 基于Ti3C2Tx薄膜基底的制备及其在多巴胺拉曼检测中的应用 |
| Preparation of Ti3C2Tx films substrate and its application in Raman detection of dopamine |
| 投稿时间：2025-01-24 |
| DOI：10.3969/j.issn.1005-5630.202501240013 |
| 中文关键词: Ti3C2Tx 表面增强拉曼散射 碱化处理 AgNPs负载量 多巴胺 |
| 英文关键词:Ti3C2Tx surface-enhanced Raman scattering alkalization treatment loading amount of AgNPs dopamine |
| 基金项目:国家自然科学基金(61875126) |
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| 中文摘要: |
| Ti3C2Tx 作为一种亲水性、生物相容性、电导性及稳定性良好的衬底，在生物传感领域有着广泛的应用前景。但纯Ti3C2Tx纳米片的灵敏度较低，且Ti3C2Tx表面具有还原性的－OH基团数量较少，所以在Ti3C2Tx表面原位生长的金属纳米粒子的负载量较低。通过碱化处理，将Ti3C2Tx表面的－F和＝O基团转化为－OH基团，有效提高了Ti3C2Tx表面的贵金属纳米颗粒密度。制得的银纳米颗粒（silver nanoparticles，AgNPs）负载的Ti3C2－OH薄膜基底，在表面增强拉曼散射（surface enhancement of Raman scattering，SERS）检测中表现出优异性能。通过控制AgNPs在Ti3C2－OH膜上的负载量，将多巴胺（dopamine，DA）的SERS检测极限提升到了1×10–9 mol/L。此外，该基底稳定均匀（相对标准偏差6.36%），针对DA的动态检测范围达到5个数量级（1×10–4～1×10–9 mol/L）。研究结果表明，Ag@Ti3C2－OH具有作为高灵敏度SERS芯片的潜能，适用于基于SERS技术的生物分子DA的无标记定量检测。 |
| 英文摘要: |
| Ti3C2Tx, with their excellent hydrophilicity, biocompatibility, electrical conductivity and high stability, have broad application prospects in the field of biosensing. However, the sensitivity of pure Ti3C2Tx nanosheets is relatively low (poor enhancement performance and low detection limit et al), and the number of reducing －OH groups on the Ti3C2Tx surface is small, resulting in a low loading capacity of metal nanoparticles grown in situ on the Ti3C2Tx surface. In this paper, through alkalization treatment, the －F and ＝O groups on the Ti3C2Tx surface were converted into －OH groups, effectively increasing the density of noble metal nanoparticles on the Ti3C2Tx surface. The silver nanoparticles (AgNPs) loaded Ti3C2－OH film substrate material prepared in this way exhibited excellent performance in surface-enhanced Raman scattering (SERS). By controlling the loading amount of AgNPs on the Ti3C2－OH film, the SERS detection limit of dopamine (DA) was improved to 1×10–9 mol/L. In addition, the substrate was uniform and stable (relative standard deviation 6.36%), and the dynamic detection range for DA reached five orders of magnitude (1×10–4 mol/L to 1×10–9 mol/L). The research results show that Ag@Ti3C2－OH has the potential to be used as a highly sensitive SERS chip and is suitable for label-free quantitative detection of biomolecules like DA based on SERS technology. |
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