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| 用于生物标志物微阵列芯片检测的高灵敏度成像系统 |
| High-sensitivity imaging system for biomarker microarray chip detection |
| 投稿时间：2025-01-22 |
| DOI：10.3969/j.issn.1005-5630.202501220012 |
| 中文关键词: 微阵列技术 蛋白质芯片 反卷积 标志物检测 |
| 英文关键词:microarray technology protein chips deconvolution marker detection |
| 基金项目:上海市科委“科技创新行动计划”项目(22140900900) |
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| 中文摘要: |
| 本文研究了一种用于生物标志物微阵列芯片检测的高灵敏度成像系统。该系统以共聚焦型光路为成像基础，结合维纳滤波反卷积算法，以及阈值背景矫正处理，有效降低了点扩散函数引起的噪声及随机噪声。该系统对阿尔茨海默病的3种生物标志物微阵列芯片的检测结果表明：其对Aβ42（β淀粉样蛋白42）、P-tau181（磷酸化tau 181 蛋白）、T-tau（总tau蛋白）的检出限分别为8.60、20.60、13.12 pg/mL，各检测曲线的决定系数R2均大于0.99，测量重复性指标小于3.8%。与博奥生物公司的共聚焦芯片扫描仪的对比测试结果显示，该系统的检测限达到了商用共聚焦芯片扫描仪的同等数量级水平，并解决了激光共聚焦扫描成像技术的高复杂性与传统CCD成像灵敏度较低的问题，在疾病现场实时检测与预防等领域中具有广阔的应用空间。 |
| 英文摘要: |
| This paper studies a highly sensitive imaging system for the detection of biomarker microarray chips. The system is mainly based on a confocal optical path for imaging, combined with the Wiener filter deconvolution algorithm and threshold background correction processing, effectively reducing the noise caused by the point spread function and random noise. Through the detection of three biomarker microarray chips for Alzheimer's disease, the detection limits of Aβ42 (beta-amyloid protein 42), P-tau181 (phosphorylated tau 181 protein), and T-tau (total tau protein) were determined to be 8.60, 20.60, and 13.12 pg/mL, respectively. The correlation coefficients R2 of the curves were all greater than 0.99, and the measurement repeatability index was less than 3.8%. Comparative tests with the confocal chip scanner from BGI showed that the detection limit of the biomarker microarray chip imaging system reached the same order of magnitude as commercial confocal chip scanners, and it overcame the high complexity of laser confocal scanning imaging technology and the low sensitivity of traditional CCD imaging. It has broad application prospects in the fields such as real-time on-site detection and prevention of diseases. |
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