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| 基于Au@Ag 纳米粒子的氨基酸表面增强拉曼散射检测 |
| Surface-enhanced Raman scattering detection of amino acids based on Au@Ag nanoparticles |
| 投稿时间：2024-03-01 |
| DOI：10.3969/j.issn.1005-5630.202403010029 |
| 中文关键词: 氨基酸 表面增强拉曼散射 金核银壳纳米粒子 定量检测 |
| 英文关键词:amino acid surface-enhanced Raman scattering Au@Ag NPs quantitative detection |
| 基金项目:国家自然科学基金（62275156) |
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| 中文摘要: |
| 表面增强拉曼散射（surface-enhanced Raman scattering，SERS）因具有高灵敏度的分子特征提取能力和多重检测优势，被广泛用于氨基酸定量分析。稳定且高灵敏度的SERS基底是实现精准检测的重要基础。金核银壳纳米粒子（gold core silver shell nanoparticles，Au@Ag NPs）兼具金纳米粒子（gold nanoparticles，Au NPs）的化学稳定性和银纳米粒子（silver nanoparticles，Ag NPs）的高SERS增强效应，可实现对苯丙氨酸、丙氨酸等7种氨基酸的筛选检测。除异亮氨酸和甘氨酸灵敏度较低外，其余氨基酸的检测限可达1×10–4 mol/L，且检测浓度与SERS 信号强度呈线性相关（相关系数均大于0.94）。该研究为同一基底定量检测多重氨基酸提供了参考方案。 |
| 英文摘要: |
| Surface-enhanced Raman scattering (SERS) is widely employed for amino acids quantitative analysis due to its high-sensitivity molecular feature extraction and multiplex detection advantage. Stable and highly sensitive SERS substrates are crucial for achieving precise detection. Gold core silver shell nanoparticles (Au@Ag NPs) combine the chemical stability of gold nanoparticles with the high SERS enhancement effect of silver nanoparticles. They enable the screening and detection of seven amino acids, including phenylalanine and alanine. Except for isoleucine and glycine, which exhibited relatively lower sensitivity, the detection limits for the remaining amino acids reached 1×10–4 $ \mathrm{m}\mathrm{o}\mathrm{l}/\mathrm{L} $. A linear correlation existed between concentration and SERS signal intensity, with correlation coefficients exceeding 0.94. This study provides a reference method for simultaneous quantitative detection of multiple amino acids on a single substrate. |
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