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期刊信息
  • 主管单位:
  • 中国科学技术协会
  • 主办单位:
  • 中国仪器仪表学会、上海光学仪器研究所、中国光学学会工程光学专业委员会
  • 主  编:
  • 庄松林
  • 地  址:
  • 上海市军工路516号上海理工大学《光学仪器》编辑部
  • 邮政编码:
  • 200093
  • 联系电话:
  • 021-55270110
  • 电子邮件:
  • gxyq@usst.edu.cn
  • 国际标准刊号:
  • 1005-5630
  • 国内统一刊号:
  • 31-1504/TH
  • 邮发代号:
  • 单  价:
  • 15.00
  • 定  价:
  • 90.00
三维培养结肠癌细胞的微流控芯片荧光成像研究
Investigation of fluorescence imaging in microfluidic chip for three-dimensional cultivation of colon cancer cells
投稿时间:2023-03-11  
DOI:10.3969/j.issn.1005-5630.202303110052
中文关键词:  微流控芯片  三维培养  荧光成像  荧光蛋白
英文关键词:microfluidic chip  three-dimensional cultiviation  fluorescence imaging  fluorescent protein
基金项目:上海市军民融合发展专项资金科技创新支持项目 (2020-jmrh-kj8))
作者单位E-mail
蔡书祺 上海理工大学 光电信息与计算机工程学院上海 200093  
郑璐璐 上海理工大学 光电信息与计算机工程学院上海 200093 llzheng@usst.edu.cn 
张大伟 上海理工大学 光电信息与计算机工程学院上海 200093  
摘要点击次数: 140
全文下载次数: 109
中文摘要:
      改良了一种微流控芯片,可用于对结肠癌细胞进行三维培养并实现实时荧光成像。在结肠癌细胞内植入内源性的红色荧光蛋白,使用激光共聚焦显微镜对芯片中三维培养的细胞进行成像。通过细胞内部红色荧光蛋白的表达,可以观测到细胞的生长状态,实现对细胞的实时监测和高分辨率荧光成像。同时,通过免疫荧光染色来表征反映细胞活性的特征蛋白,其荧光强度和蛋白表达呈正相关。研究结果提示,细胞活性相关蛋白的表达受到微环境的影响,其在芯片三维培养中的活性强于二维培养,表明芯片内环境更加接近真实的人体微环境。该方法为进一步探究肿瘤细胞转移机制及相关药物的筛选研究提供了一种新的技术手段及实验平台。
英文摘要:
      This article presents an improved method for the three-dimensional cultivation of colon cancer cells and the realization of real-time fluorescence imaging using a microfluidic chip. By incorporating endogenous red fluorescent protein into the colon cancer cells, we employed laser confocal microscopy to visualize the cells cultivated within the chip in three dimensions. The expression of intracellular red fluorescent protein allowed for the observation of cell growth status, facilitating real-time monitoring and high-resolution fluorescence imaging. Moreover, immunofluorescence staining was employed to characterize feature proteins indicative of cellular activity, with their fluorescence intensity demonstrating a positive correlation with protein expression. The research findings indicate that the expression of activity-related proteins is influenced by the microenvironment, exhibiting stronger activity in the three-dimensional cultivation on the chip compared to the two-dimensional approach. This suggests that the microenvironment within the chip more closely resembles the actual microenvironment within the human body. This method provides a novel technical approach and experimental platform for further exploration of tumor metastasis mechanisms and the screening of related drugs.
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