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| 光激活膀胱癌焦亡的新型药物的研究 |
| Research of the novel drug for photoactivated pyroptosis of bladder cancer |
| 投稿时间：2023-02-28 |
| DOI：10.3969/j.issn.1005-5630.202302280032 |
| 中文关键词: 吲哚菁绿 膀胱癌 去甲基化 细胞焦亡 |
| 英文关键词:indocyanine green bladder cancer demethylation pyroptosis |
| 基金项目:上海市军民融合发展专项资金科技创新支持项目(2020-jmrh-kj8) |
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| 中文摘要: |
| 设计了一种利用牛血清蛋白(bovine serum albumin，BSA)包裹吲哚菁绿(indocyanine green，ICG)和RG108的纳米材料ICG/RG108@BSA。吲哚菁绿在近红外激光诱导下可以活化Caspase-3蛋白，RG108通过抑制DNA甲基化来上调GSDME蛋白表达，从而增强了Caspase-3蛋白切割GSDME蛋白引起的膀胱癌细胞焦亡。ICG/RG108@BSA具有优异的生物相容性，能够被膀胱癌细胞有效吞噬。ICG/RG108@BSA在755 nm激光激活下，会对膀胱癌细胞产生明显的杀伤效果，其中小鼠膀胱癌细胞Mb49的存活率仅为6.9%，人膀胱移行细胞癌细胞T24的存活率仅为10.7%。同时755 nm激光激发的ICG/RG108@BSA材料也成功诱导了膀胱癌细胞焦亡，为膀胱癌的肿瘤免疫治疗提供了有利的条件。 |
| 英文摘要: |
| In this paper, a nanomaterial named ICG/RG108@BSA was designed to encapsulate indocyanine green (ICG) and RG108 using bovine serum protein (BSA). Indocyanine green activated protein Caspase-3 under near infrared laser induction and RG108 up-regulated the expression of GSDME by inhibiting DNA methylation, thus enhancing the pyroptosis of bladder cancer cells caused by Caspase-3 cleavage. ICG/RG108@BSA had excellent biocompatibility, which was effectively phagocytosed by bladder cancer cells. ICG/RG108@BSA produced significant killing effect on bladder cancer cells when activated by 755 nm laser, in which the survival rate of Mb49 was only 6.9% and the survival rate of T24 cells was only 10.7%. The 755 nm laser-excited ICG/RG108@BSA nanomaterial also successfully induced bladder cancer cell pyroptosis, providing a favorable therapeutic condition for tumor immunotherapy of bladder cancer. |
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