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| 基于微流控芯片的肿瘤细胞球三维培养及药物筛选研究 |
| Research on three-dimensional culture and drug screening of tumor cell spheres based on microfluidic chips |
| 投稿时间：2023-12-19 |
| DOI：10.3969/j.issn.1005-5630.202312190137 |
| 中文关键词: 微流控芯片 三维培养 药物筛选 |
| 英文关键词:microfluidic chip three-dimensional culture drug screening |
| 基金项目:上海市科委科技创新行动计划项目（22140900900，23141901300） |
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| 中文摘要: |
| 三维培养肿瘤细胞球较真实地模拟了人体内肿瘤细胞的生长环境，可用于药物筛选及其他相关研究。结合微流控技术，三维细胞培养有了更多的实现方式。设计了一种高通量的三维微流控芯片，基于微腔阵列实现了肿瘤细胞球的快速培养及药物的快速筛选。与目前主流的三维培养需要4 d时间相比，采用微流控芯片培养则只需要15 h就可形成肿瘤细胞球，大幅度节省了培养时间。利用肿瘤生长形态监测及荧光成像技术，验证了用微流控芯片进行三维培养的可靠性。同时，基于微流控芯片还实现了多种药物的快速筛选，且结果均呈现出良好的浓度依赖性，由此证实了在微流控芯片中进行药物筛选的可行性。该高通量微流控芯片可应用于肿瘤学等研究领域，为实现肿瘤药物的快速筛选提供了一种新的技术手段及实验平台。 |
| 英文摘要: |
| Three-dimensional culture of tumor cell spheres can truly simulate the growth environment of tumor cells in vivo, and thus be more suitable for drug screening and other related research. Combined with microfluidic technology, there are more ways to implement three-dimensional cell culture technology. In this study, a microfluidic chip for high-throughput three-dimensional tumor cell spheres culture was designed to enable rapid cultivation and drug screening of tumor cell spheres based on microcavity arrays. Compared to the current mainstream three-dimensional culture that takes 4 days, microfluidic chips only require 15 h to form tumor spheres. It significantly saves cultivation time. The reliability of three-dimensional culture in the chip was verified by tumor growth morphology monitoring and fluorescence imaging technology. Meanwhile, this research realized multi-drug rapid screening based on chip, presented good gradient dependence and verified the feasibility of drug screening in chip. This high-throughput microfluidic chip can be applied in multiple research fields such as oncology, providing a new technological means and experimental platform for achieving rapid drug screening in tumors. |
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