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| 全波段无光学滤波显微镜的研究 |
| A study of full-spectrum filterless microscopy |
| 投稿时间：2023-12-01 |
| DOI：10.3969/j.issn.1005-5630.202312010129 |
| 中文关键词: 荧光成像 光学滤波 全波段荧光 |
| 英文关键词:fluorescence imaging optical filtering full-band fluorescence |
| 基金项目:国家重点研发计划（2022YFC3103003） |
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| 摘要点击次数: 113 |
| 全文下载次数: 262 |
| 中文摘要: |
| 传统显微镜在生物成像中广泛采用荧光成像技术，该技术在基础科学和临床研究中具有重要意义。然而，传统荧光检测系统通常依赖光谱滤波器来分离荧光信号和激发光，这导致检测速度和灵敏度下降，同时限制了可检测的荧光范围，增加了系统的光学复杂性。为解决这一问题，展示了两种全波段无滤波显微成像技术。第一种技术利用超快超连续白光源，无需光谱滤波即可激发荧光，通过时间分辨探测器在时间域中排除激发光，从而提高了检测速度和灵敏度。第二种技术利用荧光发射的偏振性和相干性，采用线偏振照明和交叉分析器，增强了荧光和散射光之间的对比度，实现了在450~680 nm光谱范围内的荧光成像。这两种全波段无滤波显微成像技术简化了光学配置，为生物成像领域的应用提供了高效、灵敏的解决方案。 |
| 英文摘要: |
| Traditional fluorescence microscopy extensively employs fluorescence imaging techniques in biological imaging, playing a crucial role in fundamental scientific research and clinical studies. However, conventional fluorescence microscopic systems often rely on spectral filters to separate fluorescence signals from excitation light, resulting in degradation of detection speed and sensitivity as well as complexity of the systems. To address this issue, we introduce two full-spectrum filterless microscopy imaging techniques. The first technique utilizes an ultrafast and broadband white light source, enabling fluorescence excitation without the need for spectral filtering. A time-resolved detector is employed to exclude excitation light in the time domain, thereby enhancing detection speed and sensitivity. The second technique exploits the polarization and coherence of fluorescence emission, employing linearly polarized illumination and a cross analyzer to improve the contrast between fluorescence and scattering light, facilitating fluorescence image acquisition within the 450-680 nm spectral range. These two full-spectrum filterless microscopy techniques offer efficient and sensitive solutions for the field of biological imaging while simplifying optical configurations. |
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