|
| 双光子受激发射损耗复合显微成像技术研究 |
| Two-photon stimulated emission depletion composite microscopy |
| 投稿时间：2020-03-11 |
| DOI：10.3969/j.issn.1005-5630.2020.04.011 |
| 中文关键词: 荧光显微 双光子显微 超高分辨率 受激发射损耗 扫描显微 |
| 英文关键词:fluorescence microscopy two-photon microscopy super resolution stimulated emission depletion scanning microscopy |
| 基金项目:国家重点研发计划（2017YFC0110305）；江苏省自然科学基金（BK20170390）；江苏省博士后基金（2018K114C） |
|
| 摘要点击次数: 2539 |
| 全文下载次数: 1919 |
| 中文摘要: |
| 鉴于双光子受激发射损耗（STED）复合显微镜在神经疾病临床诊断及脑科学研究中的重要作用，对双光子STED复合显微成像中多波长选通、多光束合束、关键技术指标等进行了研究，完成了复合显微镜样机系统集成研制和复合成像。该复合显微镜可以对荧光标记的样本进行扫描成像，具备红绿双色荧光扫描成像功能、双光子绿色荧光成像功能和STED超分辨绿色荧光成像功能。测试结果表明，该复合显微镜成像深度达到700 μm，分辨率优于60 nm。 |
| 英文摘要: |
| Two-photon (2P) stimulated emission depletion (STED) composite microscope has the potential to be widely used in clinical diagnosis of neurological diseases and brain science research. In this paper, based on the research of multi wavelengths selection, multi beams combination, key technical indexes testing research, the integrated development of composite microscopy prototype system and composite imaging are completed. The composite microscope can image the fluorescent labeled samples, which has the functions of red-green two-color fluorescence imaging, 2P green fluorescence imaging and STED super-resolution green fluorescence imaging. In terms of index, the imaging depth is 700 μm, and the resolution of STED imaging is better than 60 nm. |
| HTML 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
