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| 高通量dPCR基因芯片荧光激发测控系统的研制 |
| Development of high throughput dPCR gene chip fluorescence excitation measurement and control system |
| 投稿时间：2019-01-21 |
| DOI：10.3969/j.issn.1005-5630.2019.06.012 |
| 中文关键词: 数字聚合酶链式反应（dPCR） LED光源 荧光激发 测控系统 |
| 英文关键词:digital polymerase chain reaction the LED light source fluorescence excitation measurement and control system |
| 基金项目:上海市科学技术委员会地方院校能力建设项目（18060502500） |
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| 中文摘要: |
| 为了解决高通量数字聚合酶链式反应（dPCR）基因芯片荧光激发问题，设计了一套荧光激发测控系统。系统根据荧光染料的激发光谱特性，选择大功率窄带LED作为荧光激发光源，并通过STM32微处理器系统进行控制。该荧光激发系统有FAM、HEX和ROX三个荧光激发通道，各通道LED激发功率能够分别调节。系统最大输出电流为8 A，单通道最大输出功率为3 W，调节精确度<1.0%，系统一次可完成dPCR基因芯片9 600个微液滴的荧光激发和采集。测试实验结果表明，系统达到了设计指标和应用需求。 |
| 英文摘要: |
| In order to solve the fluorescence excitation problem of high throughput dPCR gene chip, a fluorescence excitation measurement and control system was designed in this paper. According to the excitation spectrum characteristics of fluorescent dyes, high-power narrow-band LED is selected as the fluorescent excitation light source and controlled by STM32 microprocessor system. The fluorescence excitation system has three fluorescence excitation channels including FAM, HEX and ROX, and the excitation power of each LED channel can be adjusted respectively. The maximum output current of the system is 8 A, the maximum output power of the single channel is 3 W, and the adjustment accuracy is less than 1.0%. The system could complete the fluorescence excitation and collection of 9 600 microdroplets of dPCR gene chip at one time. The experimental results show that the system meets the requirements of design and application. |
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