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| 基于二氨基荧光素类荧光探针的新型一氧化氮检测方法 |
| A creative method for NO detection based on DAF-FM |
| 投稿时间：2018-04-19 |
| DOI：10.3969/j.issn.1005-5630.2019.02.003 |
| 中文关键词: 一氧化氮(NO)检测 纳米金颗粒 荧光光谱 |
| 英文关键词:nitric oxide(NO) detection gold nanoparticles fluorescence spectroscopy |
| 基金项目:国家自然科学基金（61675133、11561121003）；上海市自然科学基金（17ZR1447500） |
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| 中文摘要: |
| 提出了一种基于二氨基荧光素类一氧化氮（NO）探针DAF-FM的新型高效NO检测方法。通过纳米金颗粒的添加，显著增强了探针荧光强度，同时NO检测的灵敏度得到了显著的提高。该新型荧光探针可重复使用24次以上，弥补了DAF-FM的单次使用缺陷，实现了一氧化氮的可持续重复检测。 |
| 英文摘要: |
| A new and efficient nitric oxide (NO) detection method based on the DAF-FM which is a kind of NO probes was proposed. The incorporation of gold nanoparticles significantly increased the fluorescence intensity of the fluorescent probe after reacting with NO. The new fluorescent probes composed of DAF-FM and gold nanoparticles have dozens of times higher fluorescence intensity than that without gold nanoparticles does. The sensitivity of NO detection has been significantly improved. The new fluorescent probe can be reused for more than 24 times, overcoming the single-use problem of DAF-FM and achieving the sustainable reuse of commercial nitric oxide detection. |
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