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| 浅谈共聚焦显微技术 |
| A brief discussion on confocal microscopy techniques |
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| DOI： |
| 中文关键词: 显微 共聚焦显微技术 激光扫描共聚焦 碟片共聚焦 结构光 |
| 英文关键词:microscopy confocal microscopy laser scanning spinning disk structured illumination |
| 基金项目: |
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| 摘要点击次数: 5475 |
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| 中文摘要: |
| 共聚焦显微镜以其高对比度、高分辨率及可重建三维图像的独特优势,在生物医学研究、微细加工、半导体和高分子材料的生产检测等领域获得广泛应用。常用的共聚焦技术方法有:传统的激光扫描共聚焦显微镜(LSCM),其特点是获得的图像对比度和分辨率高,但需要逐点扫描,帧成像时间长,系统复杂,体积大,价格昂贵;碟片共聚焦显微镜(SDCM)是采用多光束扫描的方法来获得共聚焦图像,速度可以大大提高,但牺牲了共聚焦图像的分辨率,系统更为复杂,且不能调整轴向分辨率;结构光显微镜(SIM)具有方法简单,可模块化设计,成本低,成像质量接近于激光扫描共聚焦显微镜,成像速度快,性价比较高。 |
| 英文摘要: |
| Confocal microscopy boasts such advantages as high contrast, high resolution and easy to 3D reconstruction. Therefore, the device has been widely applied in the manufacture and detection of biology and medical research, micro machining, semiconductor and macromolecule. There are several kinds of confocal microscopy, including laser scanning confocal microscopy(LSCM), spinning disk confocal microscopy (SDCM) and structured illumination microscopy (SIM). LSCM has such advantages as high contrast and resolution, but it is complicated, bulky, expensive and slow in imaging. SDCM can get the confocal image fast based on the lower resolution and more complicated technique. Though SIM is the simplest and cheapest confocal microscopy, it can offer high quality image with fast speed. |
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