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| SPR生化分析仪及其在样品检测中的应用 |
| Surface plasmon resonance biochemical analysis instrument andits application in the samples concentration detection |
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| DOI： |
| 中文关键词: 表面等离子共振 SPR生化分析仪 检测 |
| 英文关键词:surface plasmon resonance SPR biochemical analysis instrument detection |
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| 中文摘要: |
| 表面等离子共振(SPR)是一种入射光照射到金属表面,导致金属表面产生等离子共振,反射光强出现衰减的现象,反射光强衰减程度与入射光波长、入射角及金属表面补测物质的折射率有关。根据这原理研制的SPR生化分析仪,在检测、分析分子间的相互作用等方面有着广泛的应用。针对HPSPR 6000生化分析仪的工作原理、技术参数、检测方法进行了详细的介绍,在芯片表面生长磺胺二甲嘧啶 BSA,并检测生长过程的响应信号,同时还对磺胺二甲嘧啶抗原与抗体在芯片表面的结合响应信号进行了检测。 |
| 英文摘要: |
| Surface plasmon resonance (SPR) is a phenomenon which occurs when light is reflected off thin metal films, and the intensity of reflected light is correlated with the optical wavelength of incidence, angle of incidence, and refractive index of the material on the surface of Au film. Biochemical analysis instruments based on surface plasmon resonance have been developed for characterizing and quantifying biomolecular interaction. The working principle, technical parameters and testing method of the HPSPR 6000 were discussed here. The SPR response of immobilizating sulfamethazine antign on the surface of chip was tested and the binding value between antign and antibody on the surface chip was measured. |
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